For the first time the LaserLab group has had two articles accepted on the same day! Both are in ACS Analytical Chemistry and both are on the use of our fully-programmable, broadband light source for highly multiplexed fluorescence spectroscopy.
Recent graduate Dr Travis Ferguson demonstrates in “Rapid Fluorescence EEM Spectroscopy Using Super-Cycle Hadamard-Transform Multiplexing” (link) how his clever algorithm can make fluorescence excitation-emission-matrix (EEM) spectra 10-100 times faster than previously thought possible.
The same light source was used by recent graduate Oren Katz and PhD-candidate Emma Abbey to perform Fluorescence Excitation-Emission Matrix Imaging (link). They used the multiplexed light source to excite the fluorescence of a spatially inhomogeneous sample and used a hyperspectral camera to obtain over 65,000 EEM spectra in one image frame, i.e. one EEM per pixel. With an acquisition time of under 10 seconds we can record 5-dimensional data cubes (EEM-movies).that allow us to perform a chemical analysis on each pixel.